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US 2014/0231710 A1Published application
Method of making a formulation for deactivating nucleic acids
AssigneeGen-Probe Incorporated
InventorsNorman C. NELSON, Kenneth A. BROWNE, Lizhong DAI, James RUSSELL, Mark E. FILIPOWSKY, Margarita B. KAMINSKY, Daniel L. KACIAN
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Abstract
The disclosure relates to formulations for use in deactivating nucleic acids and methods of making and using the same.
Claims19
A method of making a formulation for deactivating nucleic acids, the method comprising the steps of:
- (a) separately dissolving solid forms of a corrosion-inhibiting agent and a wetting agent, the corrosion-inhibiting agent being phosphate, borate or sodium bicarbonate, and the wetting agent being a surfactant or detergent;
- (b) combining together the dissolved forms of the corrosion-inhibiting agent and the wetting agent to form a first mixture;
- (c) combining together the first mixture with a solubilizing agent to form a second mixture, the solubilizing agent being an organic solvent or an emulsifying agent;
- and
- (d) instructing a user to combine the second mixture with a deactivating agent to form the formulation, the deactivating agent being sodium hypochlorite or dichloroisocyanurate,
- whereby the concentration of the corrosion-inhibiting agent in the formulation is from about 10 mM to about 750 mM;
- whereby the concentration of the wetting agent in the formulation is from about 0.005% to about 1% (w/v);
- whereby the concentration of the solubilizing agent in the formulation is from about 0.001% to about 20% (v/v);
- whereby the concentration of the deactivating agent in the formulation is from about 0.06% to about 3% (w/v) if the deactivating agent is sodium hypochlorite;
- whereby the concentration of the deactivating agent in the formulation is from about 5 mM to about 400 mM if the deactivating agent is dichloroisocyanurate;
- and
- whereby each agent of the formulation will remain substantially in solution at 22° C.
9 more elements
Dependencies are inferred from the claim text.