System and method to separate cells and/or particles
AssigneeAcousort AB
InventorsThomas Laurell, Carl Grenvall, Cecilia Magnusson, Per Augustsson
Life of this patent
Estimating the term…
Abstract
The disclosure related to a method and system to separate a subgroup of objects from a mixture of objects present in a suspension wherein said suspension if exposed to acoustic forces acting in one or two dimensions, which forces said objects to separate from each other into groups which have similar properties in connection with size and/or mass density and/or compressibility.
Claims9
A micro scaled method for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension, comprising the steps of;
- i) subjecting a suspension to pressure, wherein said pressure forces said suspension into one inlet and into at least one pre-alignment channel present on a microfluid chip,
- ii) subjecting said suspension to a two dimensional acoustic force directed perpendicular to the length direction of the pre-alignment channel,
- iii) forcing said mixture of cells and/or particles and infusing cell free buffer solution through a second inlet simultaneously into at least one separation channel, wherein said mixture of cells and/or particles are forced towards the walls of the channel,
- iv) subjecting said cells and/or particles to a one dimensional acoustic force directed perpendicular to the length direction of the separation channel and
- v) collecting cells and/or particles having the same size and/or mass density and/or compressibility from said second channel present on the microfluid chip through at least two outlets.
A micro system for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension, comprising;
- i) a micro chip having at least one pre-alignment channel and at least one separation channel, with at least two inlets and at least two outlets,
- ii) means for providing pressure, wherein said pressure, forces said suspension into the first inlet,
- iii) means for providing a two dimensional acoustic force, wherein said two dimensional acoustic force is directed perpendicular to said pre-alignment channel
- iv) means for infusing cell free buffer in said second inlet,
- v) means for providing a one dimensional acoustic force, wherein said one dimensional acoustic force is directed perpendicular to said separation channel and
- vi) means for collecting cells and particles, having the same size and/or mass density and/or compressibility, from said channel.
Dependencies are inferred from the claim text.