Methods and materials for obtaining induced pluripotent stem cells
AssigneeMayo Foundation for Medical Education and Research
InventorsTimothy J. Nelson, Andre Terzic, Almudena J. Martinez Fernandez, Clifford D. Folmes
Life of this patent
Estimating the term…
Abstract
This document provides methods and materials involved obtaining induced pluripotent stem (iPS) cells. For example, methods and materials for increasing the efficiency for making iPS cells as well as methods and materials for selecting iPS cells are provided.
Claims22
A method for augmenting production of induced pluripotent stem cells being produced from somatic cells by the expression of stemness transcription factors, wherein said method comprises contacting said somatic cells with an effective amount of an agent that promotes or aids glycolysis.
A method for identifying induced pluripotent stem cells within a sample comprising a mixture of somatic cells and said induced pluripotent stem cells, wherein said method comprises:
- (a) contacting said sample with a marker of glycolysis, wherein said marker labels said induced pluripotent stem cells to a greater extent than said somatic cells, and
- (b) obtaining said induced pluripotent stem cells from said mixture based at least in part on an increased level of said marker being associated with said induced pluripotent stem cells.
11-12. (canceled)
A method for identifying induced pluripotent stem cells within a sample comprising a mixture of somatic cells and said induced pluripotent stem cells, wherein said method comprises:
- (a) contacting said sample with a marker of mitochondrial membrane potential, wherein said marker labels said induced pluripotent stem cells to a greater extent than said somatic cells, and
- (b) obtaining said induced pluripotent stem cells from said mixture based at least in part on an increased level of said marker being associated with said induced pluripotent stem cells.
19-22. (canceled)
Dependencies are inferred from the claim text.