Back to results
US 2014/0212886 A1Published application
Shuttle vectors for corynebacterium and escherichia coli
AssigneeSamsung Electronics Co., Ltd.
InventorsWoo-yong LEE, Young-min Lee, Jae-chan Park, Jin-hwan Park, Yeo-ju Song
Life of this patent
Estimating the term…
Abstract
A vector that allows for easy accomplishment of a variety of cloning, and use of the vector for measurement of the transcription-inducing activity of a promoter or production of a desired gene product in Corynebacterium.
Claims16
A vector comprising a nucleotide sequence of SEQ ID NO:
- 1 and a multi-cloning site (MCS) comprising a nucleotide sequence of SEQ ID NO:
- 2.
A method of examining promoter activity, the method comprising:
- culturing a Corynebacterium comprising a vector in a medium, wherein the vector comprises a nucleotide sequence of SEQ ID NO:
- 1, a MCS sequence of SEQ ID NO:
- 2, a reporter gene and a transcription terminator operably linked in the MCS, and a promoter in the MCS, whereby a product of the reporter gene is expressed;
- measuring the product of the reporter gene;
- and
- examining the transcription-inducing activity of the promoter based on the measurement.
3 more elements
A method of producing a gene product in Corynebacterium, the method comprising:
- culturing a Corynebacterium comprising a vector in a medium, wherein the vector comprises a nucleotide sequence of SEQ ID NO:
- 1 and a MCS sequence of SEQ ID NO:
- 2;
- a constitutive promoter, a 3′UTR and a transcription terminator operably linked in the MCS;
- and a gene in the MCS, whereby a gene product of the gene is produced.
2 more elements
Dependencies are inferred from the claim text.