Endogenous dnase activity to reduce dna content
AssigneeDanisco US Inc.
InventorsKatherine M. Hoffmann, Douglas Ko, Michael Ward
Life of this patent
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Abstract
The application provides a method of reducing the DNA content of a protein preparation or a culture broth from a filamentous fungal host cell using an endogenous filamentous fungal host DNase activity.
Claims27
A method of reducing DNA content of a broth in which filamentous fungal host cells has been cultured, comprising the steps of:
- adjusting the pH and/or temperature of a broth in which the fungal host cells have been cultured for at least 24 hours to increase the pH and/or the temperature used in culturing;
- and
- incubating the broth for a sufficient period at the increased pH and/or temperature to detectably reduce fungal host DNA in the preparation;
- provided that the reduction of DNA is not primarily due to presence of an exogenous DNase in the broth.
A method of reducing DNA content of a protein preparation from filamentous fungal host cells, comprising the steps of:
- assessing a level of filamentous fungal host cell DNA in a protein preparation from the fungal host cells;
- adjusting the pH and/or temperature of the protein preparation to an adjusted pH and/or temperature;
- incubating the protein preparation for a sufficient period at the adjusted pH and/or temperature to detectably reduce the level of filamentous fungal host cell DNA in the protein preparation;
- and
- determining a reduction of the amount of filamentous fungal host cell DNA in the protein preparation;
- provided that the reduction is not primarily due to an exogenous DNase present in the protein preparation.
A method of reducing DNA content of a broth in which a filamentous fungal host cells have been cultured, comprising the steps of:
- increasing the pH and/or temperature of a broth in which the filamentous fungal host cells have been cultured for at least 24 hr, and on which a solid-liquid separation step has been performed to separate the broth from filamentous fungal host cells;
- and
- incubating the broth for a sufficient period at the increased pH and/or temperature to detectably reduce filamentous fungal host DNA in the broth;
- provided that the reduction is not primarily due to an exogenous DNase present in the broth.
Use of an endogenous filamentous fungal host cell DNase activity to reduce filamentous fungal host DNA in a protein preparation from the filamentous fungal host cell.
Use of an endogenous filamentous fungal host cell DNase activity to reduce filamentous fugal host DNA in a culture broth in which the filamentous fungal host cell has been cultured.
Dependencies are inferred from the claim text.