Compositions, organisms, systems, and methods for expressing a gene product in plants

A plant comprising a first expression vector and a second expression vector,

• wherein the first expression vector comprises
• a first promoter,
• a first copy of an coding sequence operably linked to the first promoter;
• and
• a first 3′ termination sequence operably linked to the first copy of the coding sequence, the first promoter having sufficient promoter activity to express the first copy of the coding sequence in the plant cell, and
• wherein the second expression vector comprises
• a second promoter,
• a second copy of the coding sequence operably linked to the second promoter;
• and
• a second 3′ termination sequence operably linked to the second copy of the coding sequence, the second promoter having sufficient promoter activity to express the second copy of the coding sequence in the plant cell.

A method for expressing an coding sequence in a plant, the method comprising:

• contacting the cytosol of a cell of the plant with a first expression vector and a second expression vector,
• wherein the first expression vector comprises
• a first promoter,
• a first copy of an coding sequence operably linked to the first promoter;
• and
• a first 3′ termination sequence operably linked to the first copy of the coding sequence, the first promoter having sufficient promoter activity to express the first copy of the coding sequence in the plant cell, and
• wherein the second expression vector comprises
• a second promoter,
• a second copy of the coding sequence operably linked to the second promoter;
• and
• a second 3′ termination sequence operably linked to the second copy of the coding sequence, the second promoter having sufficient promoter activity to express the second copy of the coding sequence in the plant cell.

A method of directing ectopic expression of a coding sequence in a plant comprising:

• transforming the plant with a first expression cassette and a second expression cassette,
• wherein the first expression cassette comprises
• a first expression control sequence,
• a first copy of the expressible nucleic acid operably linked to the first expression control sequence;
• and
• a first 3′ termination sequence operably linked to the first copy of the expressible nucleic acid, the first expression control sequence having sufficient activity to ectopically express the first copy of the expressible nucleic acid in the plant, and
• wherein the second expression cassette comprises
• a second expression control sequence,
• a second copy of the expressible nucleic acid operably linked to the second expression control sequence;
• and
• a second 3′ termination sequence operably linked to the second copy of the expressible nucleic acid, the second expression control sequence having sufficient activity to ectopically express the second copy of the expressible nucleic acid in the plant.