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AssigneeHexima Limited
InventorsMarilyn Anne ANDERSON, Fung Tso LAY, Robyn Louise HEATH
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The present invention provides nucleic acid molecules derived from Nicotiana alata, which encode defensin-like molecules. The present invention contemplates the use of such nucleic acid molecules in the generation of transgenic plants having resistance or at least reduced sensitivity to plant pests including insects, microorganisms, fungi and/or viruses and the of the encoded defensin-like molecules in compositions for topical application to a plant or a plant part so as to reduce prevent or reduce infestation of the plant or plant part by plant pests. The transgenic plants provided by the present invention include monocotyledonous and dicotyledonous plants, and particularly include crop plants and ornamental flowering plants.
A method for generating a plant seed with increased or enhanced resistance to a plant pest, said method comprising introducing into the genome of a cell of a plant seed a genetic construct comprising a promoter or functional equivalent thereof operably linked to a nucleotide sequence encoding a floral-derived, defensin-like molecule having a mature domain comprising the amino acid sequence:—
A transfected or transformed cell of a plant seed, said cell comprising a nucleic acid molecule comprising a sequence of nucleotides encoding or complementary to a sequence encoding a polypeptide comprising, in its precursor form, an N-terminal signal domain, a mature domain and an acidic C-terminal domain wherein said polypeptide is produced during flower development and its mature domain has activity against one or more plant pests.
A method for generating a plant seed with increased or enhanced resistance to a plant pathogenic fungus or insect, said method comprising introducing into the genome of a cell from a plant seed or genome of a group of cells from a plant seed a nucleic acid molecule encoding a defensin comprising an amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:16 and SEQ ID NO:18, or an amino acid sequence set forth in any one of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:16 and SEQ ID NO:18, together with a nucleic acid molecule encoding a proteinase inhibitor.
A transfected or transformed cell from a seed from a plant, said cell comprising a nucleic acid molecule encoding a defensin, defensin-like molecule or part thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or an amino acid sequence set forth in any one of SEQ ID NO:8, SEQ ID NO:14, SEQ ID NO:16 and SEQ ID NO:18, together with a nucleic acid molecule encoding a proteinase inhibitor.
24-30. (canceled)
A composition comprising a defensin or defensin-like molecule alone or in combination with another agent.
Dependencies are inferred from the claim text.