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AssigneeMax-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.
InventorsChristoph Kirsch, Elke Logemann, Klaus Hahlbrock, Paul Rushton, Imre Somssich
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Described are synthetic promoters capable of mediating gene expression in plants upon pathogen infection. Furthermore, recombinant genes and vectors comprising said chimeric promoters as well as host cells transformed with such chimeric promoters, recombinant genes, or vectors are provided. Additionally, diagnostic compositions and kits comprising such chimeric promoters, recombinant genes, vectors or cells are described. Provided are further methods for the identification of compounds being capable of activating or inhibiting genes that are specifically expressed in plants upon pathogen infection employing the above described means. Furthermore, transgenic plant cells, plant tissue, and plants containing the above-described chimeric promoters, recombinant genes, and vectors as well as the use of the aforementioned chimeric promoters, recombinant genes, vectors and/or compounds identified by the method of the invention in plant cell and tissue culture, plant breeding, and/or agriculture are described.
A chimeric promoter capable of local gene expression in plants of an operably linked nucleic acid sequence, wherein the expression is induced by a pathogen elicitor treatment, a pathogen infection, or both, wherein the chimeric promoter comprises:
An isolated cis-acting element sufficient to direct pathogen-elicitor-specific expression, pathogen-infection-specific expression, or both, consisting of the nucleotide sequence of SEQ ID NO:
A method of rendering a gene responsive to pathogens comprising inserting at least one cis-acting element sufficient to direct pathogen elicitor-specific expression, pathogen-infection-specific expression, or both into the promoter of said gene, wherein the at least one cis-acting element comprises the nucleotide sequence of SEQ ID NO:
A method for preparing a promoter capable of mediating local gene expression in plants upon pathogen infection comprising operably linking a cis-acting element sufficient to direct elicitor-specific expression to a transcription initiation sequence of a promoter, wherein the cis-acting element comprises the nucleotide sequence of SEQ ID NO:
A promoter obtained by a method of rendering a gene responsive to pathogens comprising inserting at least one cis-acting element sufficient to direct pathogen-elicitor-specific expression, pathogen-infection-specific expression, or both, into the promoter of said gene, wherein
An isolated chimeric promoter to render a gene responsive to pathogens, obtained by a method comprising
Dependencies are inferred from the claim text.