A method for fabricating a scaffold comprising electrospun fibers using an electrospinning apparatus having a hollow needle tip, a voltage means for applying a controllable voltage to the needle tip, an electrically-grounded collector plate, and a collector plate manipulating means for controllably moving the collector plate in the orthogonal x, y, and z directions, the voltage means and the collector plate manipulating means being controllable by a computer program, said method including the steps of:
- depositing a first at least one microfiber having a diameter in the range of about 3 μm to about 30 μm onto the collector plate while electrospinning the first at least one microfiber from a polymer solution with a first voltage in the range of about 0.5 kV to about 5 kV applied to the needle tip by the voltage means and a first distance of about 0.5 mm to about 3 mm between the needle tip and the collector plate, and while moving the collector plate with the collector plate manipulating means such that the first at least one microfiber is deposited on the collector plate in an ordered pattern so as to form a first patterned microfibrous layer on the collector plate;
- moving the collector plate with the collector plate manipulating means such that the distance between the needle tip and the collector plate is increased;
- depositing a second at least one microfiber having a diameter in the range of about 3 μm to about 30 μm onto the first patterned microfibrous layer while electrospinning the second at least one microfiber from the polymer solution with a second voltage in the range of about 0.5 kV to about 5 kV applied to the needle tip by the voltage means and a second distance of about 0.5 mm to about 3 mm between the needle tip and the collector plate, and while moving the collector plate with the collector plate manipulating means such that the second at least one microfiber is deposited on the first patterned microfibrous layer in an ordered pattern so as to form a second patterned microfibrous layer on the first patterned microfibrous layer;
- moving the collector plate with the collector plate manipulating means such that the distance between the needle tip and the collector plate is increased;
- depositing at least one nanofiber having a diameter in the range of about 1 nm to about 1000 nm onto the second patterned microfibrous layer while electrospinning the at least one nanofiber from the polymer solution with a third voltage in the range of about 5 kV to about 20 kV applied to the needle tip by the voltage means and a third distance of about 8 cm to about 10 cm between the needle tip and the collector plate, such that the at least one nanofiber is deposited on the second patterned microfibrous layer so as to form a nanofibrous layer on the second patterned microfibrous layer;
- moving the collector plate with the collector plate manipulating means such that the distance between the needle tip and the collector plate is decreased;
- depositing a third at least one microfiber having a diameter in the range of about 3 μm to about 30 μm onto the nanofibrous layer while electrospinning the third at least one microfiber from the polymer solution with a fourth voltage in the range of about 0.5 kV to about 5 kV applied to the needle tip by the voltage means and a fourth distance of about 0.5 mm to about 3 mm between the needle tip and the collector plate, and while moving the collector plate with the collector plate manipulating means such that the third at least one microfiber is deposited on the nanofibrous layer in an ordered pattern so as to form a third patterned microfibrous layer on the nanofibrous layer, wherein the first, second, third and fourth voltages and the movement of the collector plate manipulating means in the aforesaid depositing and moving steps are controlled by the computer program.