Disposable capillary electrophoresis detecting device

A disposable capillary electrophoresis detecting device comprising:

• a fixing device comprising:
• a first chip-fixing base having a first chip-holding cavity horizontally arranged, the first chip-holding cavity having an end portion;
• and
• a second chip-fixing base having a second chip-holding cavity vertically arranged, the second chip-holding cavity being substantially perpendicular to the first chip-holding cavity and facing the end portion of the first chip-holding cavity;
• a capillary electrophoresis microchip horizontally placed and positioned in the first chip-holding cavity, such that an edge of the capillary electrophoresis microchip is substantially aligned to the end portion of the first chip-holding cavity, the capillary electrophoresis microchip having an injection capillary, a separation capillary and a sample tank, wherein an outlet of the separation capillary is located at the edge of the capillary electrophoresis microchip;
• and
• an electrochemical sensor microchip vertically placed and positioned in the second chip-holding cavity, the electrochemical sensor microchip comprising:
• a substrate;
• a detecting electrode located on the substrate, the detecting electrode having a sensor area;
• and
• a patterned insulation layer located on the detecting electrode, the patterned insulation layer having a thickness and exposing the sensor area, at least parts of the patterned insulation layer at least being extended to two sides of the sensor area,
• wherein when the electrochemical sensor microchip is in contact with the edge of the capillary electrophoresis microchip, the outlet of the separation capillary of the capillary electrophoresis microchip is aligned to the sensor area of the detecting electrode of the electrochemical sensor microchip, and a distance between the outlet of the separation capillary and the sensor area is the thickness of the patterned insulation layer.