Plant self nitrogen fixation by mimicking prokaryotic pathways
OwnerAdi Zaltsmaninventor
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Abstract
A method for engineering a transgenic plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria, such as cyanobacteria, or that of other bacteria, by targeting to or expressing in plant plastids bacterial nif (nitrogen fixation) genes is provided. A method for reducing the overall concentration of nitrogen in soil is provided using a plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria. Progeny of a plant that replicates the nitrogen fixation mechanism of photosynthetic bacteria is also provided.
Claims35
A transgenic plant exhibiting enhanced nitrogen fixatation produced by a method comprising the steps of:
- contacting one or more plant cells with a recombinant nucleic acid sequence encoding Nif-H, Nif-D and Nif-K genes operatively linked to a promoter sequence and a terminator sequence;
- regenerating one or more plants from the plant cells;
- and
- selecting one or more plants, cultivated from the plant cells, exhibiting enhanced nitrogen fixation, wherein said one or more plants comprise the recombinant nucleic acid sequence encoding the Nif-H, Nif-D and Nif-K genes.
A transgenic plant exhibiting enhanced nitrogen fixatation produced by a method comprising the steps of:
- contacting a plant cell with a recombinant nucleic acid sequence encoding one of SEQ. ID. NO. 1, SEQ. ID. NO. 2 and SEQ. ID. NO. 3 to produce a transgenic plant;
- regenerating one or more transgenic plants from the plant cell;
- and
- selecting one or more transgenic plants cultivated from the plant cell, wherein the selected transgenic plants each exhibit enhanced nitrogen fixation,
- wherein the selected one or more transgenic plants each comprise the recombinant nucleic acid sequence encoding SEQ. ID. No. 1, SEQ. ID. No. 2 or SEQ. ID. NO. 3.
A transgenic plant exhibiting enhanced nitrogen fixatation produced by a method comprising the steps of:
- contacting a plant cell with a recombinant nucleic acid sequence encoding one of SEQ. ID. NO. 1, SEQ. ID. NO. 2 and SEQ. ID. NO. 3 to produce a transgenic plant;
- contacting the plant cell with at least one synthetic coding DNA sequence (sCDS) optimized for tomato chloroplast expression;
- regenerating one or more transgenic plants from the plant cell;
- and
- selecting one or more transgenic plants, cultivated from the plant cell exhibiting enhanced nitrogen fixation, wherein said one or more plants comprise the recombinant nucleic acid sequence encoding SEQ. ID. NO. 1, SEQ. ID. NO. 2 or SEQ. ID. NO. 3 and the sCDS.
A transgenic plant exhibiting enhanced nitrogen fixatation produced by a method comprising the steps of:
- contacting a plant cell with a recombinant nucleic acid sequence encoding one of SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 and SEQ. ID. NO. 49 to produce a transgenic plant;
- regenerating one or more transgenic plants from the plant cell;
- and
- selecting one or more transgenic plants cultivated from the plant cell, wherein the selected plants each exhibit enhanced nitrogen fixation,
- wherein the selected one or more transgenic plants each comprise the recombinant nucleic acid sequence encoding SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 or SEQ. ID. NO. 49.
A transgenic plant exhibiting enhanced nitrogen fixatation produced by a method comprising the steps of:
- contacting one or more transgenic plant cells with a recombinant nucleic acid sequence encoding a Nif-H gene operatively linked to a first promoter;
- contacting the transgenic plant cells with a recombinant nucleic acid sequence encoding a Nif-D gene operatively linked to a second promoter;
- contacting the transgenic plant cells with a recombinant nucleic acid sequence encoding a Nif-K gene operatively linked to a third promoter;
- regenerating one or more transgenic plants from the plant cells;
- and
- selecting one or more transgenic plants cultivated from the plant cells, wherein the selected transgenic plants each exhibit enhanced nitrogen fixation,
- wherein the selected transgenic plants each comprise the recombinant nucleic acid sequence encoding the Nif-H gene, the recombinant nucleic acid sequence encoding the Nif-D gene and the recombinant nucleic acid sequence encoding the Nif-K gene.
Dependencies are inferred from the claim text.