US 2014/0196174 A1 · Development of Phytophthora Resistant Potato with Increased Yield

Abstract

The present invention relates to transgenic potato plants having an increased resistance against Phytophthora infestans and a comparable yield of potato tubers compared with the wildtype potato plants, wherein the blb1-gen and blb2-gen are integrated within a specific genetic background into the potato plant.

Claims21

Dependent claims

A Phythophthora-resistant transgenic potato plant, seed, tuber, plant cell or tissue thereof comprising a nucleic acid molecule comprising a nucleotide sequence having at least 80% identity with SEQ ID NO:

A Phythophthora-resistant transgenic potato plant, seed, tuber, plant cell or tissue thereof comprising:

a) a recombinant construct comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3;
and
b) further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 126 and 136 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 505 and 515 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 625 and 635 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 4752 and 4762 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 282 and 292 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 877 and 887 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 827 and 837 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
viii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 9905 and 9915 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19.

30 more elements

A method for providing a Phythophthora-resistant transgenic potato plant comprising the following steps:

a) introducing a recombinant nucleic acid comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3 into the genome of potato plant cells;
b) integrating said recombinant nucleic acid into the genome;
c) regenerating a plant from said plant cells;
d) selecting a plant comprising a nucleic acid comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3 and a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 126 and 136 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 505 and 515 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 625 and 635 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 4752 and 4762 basepairs, using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 282 and 292 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 877 and 887 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 827 and 837 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
viii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 9905 and 9915 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19.

33 more elements

A kit comprising the primer pairs for the detection of the specific integration place, selected from the group consisting of

SEQ ID NO:
4 and 5;
SEQ ID NO:
6 and 7;
SEQ ID NO:
8 and 9;
SEQ ID NO:
10 and 14;
SEQ ID NO:
12 and 13;
SEQ ID NO:
14 and 15;
SEQ ID NO:
16 and and/or
SEQ ID NO:
18 and 19.

13 more elements

A polynucleotide comprising:

a) a recombinant nucleic acid having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3;
and
b) further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 131 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment 510 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 630 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 4757 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 287 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 882 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 832 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 13234 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19 stably integrated into a potato plant cell nucleus.

29 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/115,711Filed 24 May 2012

Priority

EP 11167251.524 May 2011

Published

10 Jul 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8279
For stress resistance, e.g. heavy metal resistance: for biotic stress resistance, pathogen resistance, disease resistance
C12N 15/8282
For biotic stress resistance, pathogen resistance, disease resistance: for fungal resistance
C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
C12N
Microorganisms or enzymes

Abstract

Claims21

Dependent claims

A Phythophthora-resistant transgenic potato plant, seed, tuber, plant cell or tissue thereof comprising:

a) a recombinant construct comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3;
and
b) further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 126 and 136 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 505 and 515 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 625 and 635 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 4752 and 4762 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 282 and 292 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 877 and 887 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 827 and 837 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
viii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 9905 and 9915 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19.

30 more elements

A method for providing a Phythophthora-resistant transgenic potato plant comprising the following steps:

a) introducing a recombinant nucleic acid comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3 into the genome of potato plant cells;
b) integrating said recombinant nucleic acid into the genome;
c) regenerating a plant from said plant cells;
d) selecting a plant comprising a nucleic acid comprising a nucleic acid sequence having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3 and a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 126 and 136 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 505 and 515 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 625 and 635 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 4752 and 4762 basepairs, using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 282 and 292 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 877 and 887 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 827 and 837 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
viii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of between 9905 and 9915 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19.

33 more elements

A kit comprising the primer pairs for the detection of the specific integration place, selected from the group consisting of

SEQ ID NO:
4 and 5;
SEQ ID NO:
6 and 7;
SEQ ID NO:
8 and 9;
SEQ ID NO:
10 and 14;
SEQ ID NO:
12 and 13;
SEQ ID NO:
14 and 15;
SEQ ID NO:
16 and and/or
SEQ ID NO:
18 and 19.

13 more elements

A polynucleotide comprising:

a) a recombinant nucleic acid having at least 80% identity with SEQ ID NO:
2 or SEQ ID NO:
3;
and
b) further comprising a junction comprising a nucleic acid sequence selected from the group consisting of:
i) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 131 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
4 and SEQ ID NO:
5;
ii) a nucleic acid sequence that can be used to amplify a nucleotide fragment 510 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
6 and SEQ ID NO:
7;
iii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 630 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
8 and SEQ ID NO:
9;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 4757 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
10 and SEQ ID NO:
11;
and/or
iv) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 287 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
12 and SEQ ID NO:
13;
v) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 882 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
14 and SEQ ID NO:
15;
vi) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 832 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
16 and SEQ ID NO:
17;
and/or
vii) a nucleic acid sequence that can be used to amplify a nucleotide fragment of 13234 basepairs using a polymerase chain reaction with two primers having the nucleotide sequences of SEQ ID NO:
18 and SEQ ID NO:
19 stably integrated into a potato plant cell nucleus.

29 more elements

Dependencies are inferred from the claim text.

Development of Phytophthora Resistant Potato with Increased Yield

Life of this patent

Abstract

Claims21

Development of Phytophthora Resistant Potato with Increased Yield

Life of this patent

Abstract

Claims21