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US 2014/0194613 A1Published application
Use of microvesicles in diagnosis and prognosis of medical diseases and conditions
AssigneeThe General Hospital Corporation
InventorsJohan Karl Olov Skog, Xandra O. Breakefield, Dennis Brown, Kevin C. Miranda, Leileata M. Russo
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Abstract
The presently disclosed subject matter is directed to methods of aiding diagnosis, prognosis, monitoring and evaluation of a disease or other medical condition in a subject by detecting a biomarker in microvesicles isolated from a biological sample from the subject.
Claims6
(canceled)
A method of extracting RNA from a bodily fluid from a human, comprising:
- a. obtaining a bodily fluid from the human;
- b. processing the bodily fluid to exclude proteins, lipids, debris from dead cells, and other contaminants;
- c. isolating a microvesicle fraction from the bodily fluid by using ultracentrifugation or a filtration concentrator;
- d. washing the microvesicle fraction;
- and
- e. extracting RNA from the microvesicle fraction.
3 more elements
Dependencies are inferred from the claim text.