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US 2014/0194595 A1Published application
Method and Composition for Crystallizing G Protein-Coupled Receptors
AssigneeThe Board of Trustee of the Leland Stanford Junior University
InventorsBrian Kobilka, Daniel Rosenbaum
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Abstract
Certain embodiments provide a method for crystallizing a GPCR. The method may employ a fusion protein comprising: a) a first portion of a G-protein coupled receptor (GPCR), where the first portion comprises the TM1, TM2, TM3, TM4 and TM5 regions of the GPCR; b) a stable, folded protein insertion; and c) a second portion of the GPCR, where the second portion comprises the TM6 and TM7 regions of the GPCR.
Claims17
A fusion protein comprising, from N-terminus to C-terminus:
- a) a first portion of a G-protein coupled receptor (GPCR), wherein said first portion comprises TM1, TM2, TM3, TM4 and TM5 regions of said GPCR;
- b) a stable, folded protein insertion;
- c) a second portion of said GPCR, wherein said second portion comprises TM6 and TM7 regions of said GPCR;
Dependencies are inferred from the claim text.