Biomarkers for Painful Intervertebral Discs and Methods of Use Thereof
AssigneeThe Regents of the University of California
InventorsZorica Buser, Jeffrey Charles Lotz
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Abstract
The present disclosure provides methods of diagnosing painful intervertebral discs. The present disclosure also provides methods of treating a painful intervertebral disc.
Claims23
A method of identifying a painful intervertebral disc, the method comprising assaying for an expression level of a gene product of at least one pain indicator gene selected from COL2A, COL9A3, AGC1, DCN, MMP3, MMP13, TIMP1, TIMP3, CD44, HIF1, IL8, IL1R, PLA2, TSG6, MMP9, Col1A1, Col3, BMP7, TNF-α, IL1β, PGE2, HIF1A, and ADAMTS5,
- wherein a decreased expression level of a gene product selected from COL2A, COL9A3, AGC1, DCN, MMP3, MMP13, TIMP1, TIMP3, CD44, HIF1, IL8, IL1R, PLA2, TSG6, MMP9, Col1A1, and Col3 in an intervertebral disc tissue, compared to a normal control value, indicates that the intervertebral disc is painful, and wherein an increased expression level of a gene product selected from BMP7, TNF-α, IL1β, PGE2, HIF1A, and ADAMTS5 in an intervertebral disc tissue, compared to a normal control value, indicates that the intervertebral disc is painful.
A method of treating intervertebral disc pain, the method comprising administering to an individual having intervertebral disc pain an effective amount of a tumor necrosis factor-alpha-induced protein 6 (TSG6) polypeptide, a polynucleotide comprising a nucleotide sequence encoding a TSG6 polypeptide, or a small molecule agent that increases the level of a TSG6 polypeptide in a cell.
Dependencies are inferred from the claim text.