Sample preparation on a solid support
AssigneeIllumina Cambridge Limited
InventorsNiall Anthony Gormley, Geoffrey Paul Smith
Life of this patent
Estimating the term…
Abstract
Presented are methods and compositions for using immobilized transposase and a transposon end for generating an immobilized library of 5′-tagged double-stranded target DNA on a surface. The methods are useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including massively parallel DNA sequencing.
Claims20
A method of preparing an immobilized library of tagged DNA fragments comprising:
- (a) providing a solid support having transposome complexes immobilized thereon, wherein said transposome complexes comprise a transposase bound to a first polynucleotide, said first polynucleotide comprising
- (i) a 3′ portion comprising a transposon end sequence, and
- (ii) a first tag comprising a first tag domain;
- (b) applying a target DNA to the solid support under conditions whereby the target DNA is fragmented by the transposome complexes, and the 3′ transposon end sequence of the first polynucleotide is transferred to a 5′ end of at least one strand of the fragments;
- thereby producing an immobilized library of double-stranded fragments wherein at least one strand is 5′-tagged with the first tag.
A solid support having transposome complexes immobilized thereon, wherein said transposome complexes comprise a transposase bound to a first polynucleotide, said polynucleotide comprising
- (i) a 3′ portion comprising a transposon end sequence, and
- (ii) a first tag comprising a first tag domain.
Dependencies are inferred from the claim text.