Multitag sequencing ecogenomics analysis-us

A kit comprising at least five pairs of tagged forward and reverse primer pairs, the pairs disposed separately, and each forward and reverse primer comprising, in 5′ to 3′ order:

• a sequence for immobilization and/or amplification, a tag sequence, and a priming sequence targeting a variable genetic region for amplification, wherein:
• (A) the sequence for immobilization and/or amplification is the same between said primers;
• (B) the tag sequence in each forward and reverse primer pair is the same, and different from the tag sequence of the other primer pairs;
• and
• (C) the priming sequences between the primer pairs are the same, and target for amplification a variable genetic region selected from:
• a 16S rRNA sequence, an 18S rRNA sequence, an ITS sequence, a mitochondrial sequence, a microsatellite sequence, a metabolic enzyme sequence, and a variable genetic disease sequence.