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US 2014/0194317 A1Published application
Biomarkers for the detection of head and neck tumors
AssigneeLoma Linda University
InventorsPenelope J. Duerksen-Hughes, Maria Filippova, Valeri Filippov
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Abstract
A method of detecting the presence of specific human papilloma virus and host cell biomarkers associated with head and neck tumors in biological samples, like saliva, blood or biopsy tissue, obtained from a subject.
Claims40
A method of detecting biomarkers associated with head and neck tumors in a patient comprising:
- (a) providing a first patient sample from the patient, wherein the first patient sample is selected from the group consisting of saliva, whole blood, white blood cells, serum, plasma and biopsy tissue from the throat, oropharynx or mouth of the patient;
- (b) contacting the first patient sample with:
- (1) a first reagent that specifically binds to one or more than one HPV (human papillomavirus) biomarker, and allowing the first reagent to bind to the one or more than one HPV biomarker if the HPV biomarker is present in the first patient sample;
- and
- (2) a second reagent that specifically binds to one or more than one host cell biomarker, wherein the host cell biomarker is differentially expressed in head and neck tumor cells as compared to normal cells, and allowing the second reagent to bind to the one or more than one host cell biomarker;
- where the first reagent binds to the one or more than one HPV biomarker if the HPV biomarker is present in the first patient sample, and the second reagent binds to the one or more than one host cell biomarker simultaneously;
- (c) simultaneously detecting the presence or absence of the HPV biomarker in the first patient sample and determining the expression level of the host cell biomarker in the first patient sample;
- and
- (d) comparing the expression level of the host cell biomarker in the first patient sample to an expression level of the host cell biomarker from at least one reference sample, wherein the reference sample is a comparable biological sample obtained from a disease-free subject.
6 more elements
A method of detecting biomarkers associated with head and neck tumors in a patient comprising:
- (a) providing a patient sample from the patient, wherein the patient sample is selected from the group consisting of saliva, whole blood, white blood cells, serum, plasma and biopsy tissue from the throat, oropharynx or mouth of the patient;
- (b) contacting the patient sample with:
- (1) a first reagent that specifically binds to one or more than one HPV (human papillomavirus) biomarker, and allowing the first reagent to bind to the one or more than one HPV biomarker if the HPV biomarker is present in the patient sample;
- and
- (2) a second reagent that specifically binds to one or more than one host cell biomarker, wherein the host cell biomarker is differentially expressed in HPV positive head and neck tumor cells as compared to HPV negative head and neck tumor cells, and allowing the second reagent to bind to the one or more than one host cell biomarker;
- where the first reagent binds to the one or more than one HPV biomarker if the HPV biomarker is present in the patient sample, and the second reagent binds to the one or more than one host cell biomarker simultaneously;
- (c) simultaneously detecting the presence or absence of the HPV biomarker in the patient sample and determining the expression level of the host cell biomarker in the patient sample;
- and
- (d) comparing the expression level of the host cell biomarker in the patient sample to an expression level of the host cell biomarker from at least one reference sample, wherein the reference sample is a comparable biological sample obtained from a patient with an HPV negative head and neck tumor.
6 more elements
Dependencies are inferred from the claim text.