Complex sets of mirnas as non-invasive biomarkers for dilated cardiomyopathy

A kit for diagnosing of dilated cardiomyopathy comprising

• (i) means for determining an expression profile of a set comprising at least two miRNAs representative for dilated cardiomyopathy in a blood sample from a subject, comprising:
• (a) a set of at least two polynucleotides,
• wherein the polynucleotides are for detecting a set comprising at least two miRNAs for diagnosing of dilated cardiomyopathy in a blood sample from a subject and wherein the nucleotide sequences of the miRNAs comprised in the set are selected from the group consisting of SEQ ID NO:
• 1 to 387.
• (b) a biochip, a RT-PCT system, a PCR-system, a flow cytometer or a next generation sequencing system
• and
• (ii) at least one reference.
• wherein the nucleotide sequences of said miRNAs are selected from the group consisting of SEQ ID NO:
• 1 to 387, a fragment thereof, and a sequence having at least 80% sequence identity thereto

A method for diagnosing dilated cardiomyopathy and diagnosing the severity of dilated cardiomyopathy comprising the steps of:

• (i) determining an expression profile of a set comprising at least two miRNAs representative for dilated cardiomyopathy in a blood sample from a subject, and
• (ii) comparing said expression profile to a reference, wherein the comparison of said expression profile to said reference allows for diagnosing dilated cardiomyopathy and diagnosing the severity of dilated cardiomyopathy
• wherein the nucleotide sequences of the miRNAs comprised in the set are selected from the group consisting of SEQ ID NO:
• 1, 3, 6, 14, 24, 30, 34, a fragment thereof, and a sequence having at least 80% sequence identity thereto.