US 2014/0194309 A1Published application

In vitro method for predicting in vivo genotoxicity of chemical compounds

AssigneesAcademisch Ziekenhuis Maastricht, Universiteit Maastricht

InventorsJoseph Henri Marie van Delft, Joseph Catharina Stephanus Kleinjans, Christina Magkoufopoulu, Danyel Gerardus Jacobus Jennen

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Abstract

The invention is in the field of genomics and it provides an in vitro method for predicting whether a compound is genotoxic in vivo. In particular, the invention provides a method for predicting the in vivo genotoxicity of a compound comprising the steps of performing an Ames test on the compound and determining if the result is positive or negative, followed by a step wherein the gene expression of at least 3 genes is determined in a HepG2 cell, compared to a reference value and predicting that the compound is in vivo genotoxic if the expression level of more than 2 of the genes is above a reference value.

Claims9

Dependent claims

A method for predicting the in vivo genotoxicity of a compound, the method comprising:

exposing a HepG2 cell to the compound for a period of time between 12 and 48 hours,
determining, for a HepG2 cell exposed to an Ames positive compound, the level of expression of a first gene set comprising at least genes NR0B2, PWWP2B and LOC100131914,
determining, for a HepG2 cell exposed to an Ames negative compound, the level of expression of a second gene set, comprising at least genes SLC40A1, PNMA6A and C10orf65,
comparing the level of expression of the first gene set or the second gene set to a set of predetermined reference values, and
predicting the compound to be in vivo genotoxic where the expression level of at least 2 genes of the first or second gene set are above their predetermined reference values.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/117,731Filed 19 May 2012
Priority: EP 11166771.319 May 2011
Published: 10 Jul 2014
Term ends: Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12Q 1/6883
Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes: for diseases caused by alterations of genetic material
G01N 33/5014
For testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics: for testing toxicity
G01N 33/5017
For testing toxicity: for testing neoplastic activity
C12Q
Measuring or testing processes involving enzymes, nucleic acids or microorganisms

Family

US 9,822,414 B2Related patent
US 2014/0194309 A1This patent

From the US filing history. Foreign family members aren’t listed.

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