Pyrenyloxysulfonic acid fluorescent agents

A fluorescent labeled conjugate comprising:

• a component which is a member selected from an amino acid, a peptide, a protein, a polysaccharide, a nucleoside, a nucleotide, an oligonucleotide, a nucleic acid, a hapten, a psoralen, a drug, a hormone, a lipid, a lipid assembly, a synthetic polymer, a polymeric microparticle, a biological cell, a virus and combinations thereof covalently bonded to a first fluorescent moiety having the formula:
• wherein
• R and R1′ are members independently selected from H, substituted or unsubstituted alkyl and substituted or unsubstituted heteroalkyl moieties;
• X and X1 are independently selected from O, NH or S;
• s and t are independently selected from the integers from 0 to 3;
• R2 is a member selected from H, substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, and substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl;
• R3 is a member selected from substituted or unsubstituted alkyl, substituted or unsubstituted cycloalkyl, substituted or unsubstituted heteroalkyl, substituted or unsubstituted heterocycloalkyl, substituted or unsubstituted aryl and substituted or unsubstituted heteroaryl,
• wherein at least one of R2 and R3 is substituted with a member selected from a reactive group, a moiety comprising a reactive group, and a component of a conjugate, and
• R2 and R3, together with the nitrogen to which they are bound are optionally joined to form a ring which is substituted with a member selected from a reactive group, a moiety comprising a reactive group and a component of a conjugate,
• with the proviso that, when R2 is H, R3 is a cyclic structure substituted with a member selected from a reactive group, a moiety comprising a reactive group, and said component of said conjugate;
• and
• with the proviso that at least one member selected from R2 and R3 comprises at least one moiety derived from said reactive group by its reaction with a reactive moiety of said component.

A compound having structure VI:

• wherein
• designates a linker arm covalently attached to a reactive group;
• the reactive group is an activated ester of a carboxylic acid comprising an aryloxy group substituted one or more times by nitro, fluoro, chloro, cyano, trifluoromethyl, or a combination thereof;
• and
• s and t are independently selected from the integers from 0 to 3, with the proviso that at least one of s and t is at least 1.

A kit for the detection of an analyte in a sample, wherein said kit comprises a compound having structure VI:

• wherein
• s is 1;
• t is 2;
• and
• is a linker arm and the linker arm is covalently attached to a reactive group having the structure: