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AssigneeHer Majesty the Queen in Right of Canada, as Represented By the Minister of Agriculture and Agr
InventorsKevin L. Rozwadowski, Derek J. Lydiate
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The invention provides methods of modifying the level of expression or functional activity of factors such as enzymes or other catalytic proteins or structural proteins, alone or in concert, to modify the frequency of meiotic homologous recombination involving the exchange of genetic information between non-sister chromatids from homologous maternal and paternal chromosomes. The steps at which modulation may occur include: homologous chromosome pairing, double-strand break formation; resection; strand invasion; branch migration; and resolution. Methods of plant and animal breeding are also provided that utilize the modulation of meiotic homologous recombination.
A method of modulating meiotic homologous recombination in a eukaryote, comprising:
A eukaryotic cell comprising a nucleic acid encoding a protein that is an activator of meiotic homologous recombination, wherein the protein is DMC1 or RAD51, wherein the nucleic acid is operably linked to a promoter, to form a transformed eukaryotic cell capable of expressing the protein, wherein expression of the protein in the cell increases the frequency of homologous non-sister chromatid exchange during meiosis.
A eukaryotic cell comprising a nucleic acid encoding a protein that has a dominant negative effect on meiotic homologous recombination, wherein the protein is a DMC1 protein or RAD51 protein, wherein the nucleic acid is operably linked to a promoter, wherein expression of the protein in the cell decreases the frequency of homologous non-sister chromatid exchange during meiosis.
Dependencies are inferred from the claim text.