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AssigneeAgency for Science, Technology and Research
InventorsSteve Oh, Marti Lecina, Andre Choo, Shaul Reuveny, Robert Zweigert, Allen Chen
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We disclose a particle comprising a matrix coated thereon and having a positive charge, the particle being of a size to allow aggregation of primate or human stem cells attached thereto. The particle may comprise a substantially elongate, cylindrical or rod shaped particle having a longest dimension of between 50 μm and 400 μm, such as about 200 μm. It may have a cross sectional dimension of between 20 μm and 30 μm. The particle may comprise a substantially compact or spherical shaped particle having a size of between about 20 μm and about 120 μm, for example about 65 μm. We also disclose a method of propagating primate or human stem cells, the method comprising: providing first and second primate or human stem cells attached to first and second respective particles, allowing the first primate or human stem cell to contact the second primate or human stem cell to form an aggregate of cells and culturing the aggregate to propagate the primate or human stem cells for at least one passage. A method of propagating human embryonic stem cells (hESCs) in long term suspension culture using microcarriers coated in Matrigel or hyaluronic acid is also disclosed. We also disclose a method for differentiating stem cells.
A method for achieving stable, long-term culture and expansion of human pluripotent stem cells or human multipotent stem cells in suspension culture in vitro, the method comprising:
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A method of differentiating stem cells in vitro, comprising attaching human pluripotent stem cells or human multipotent stem cells to a plurality of microcarriers to form microcarrier-stem cell complexes, wherein the surface of the microcarriers is coated in a matrix comprising an extracellular matrix component, and culturing the microcarrier-stem cell complexes in suspension culture under conditions that induce the differentiation of the stem cells.
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An aggregate comprising two or more microcarriers having human pluripotent or human multipotent cells attached thereto, wherein each microcarrier is suitable for use in the growth and/or differentiation of human pluripotent or human multipotent cells in in vitro suspension culture, wherein the microcarrier comprises one or more of cellulose, dextran, hydroxylated methacrylate, or collagen, wherein the surface of the microcarrier is coated in a matrix, and wherein one or a plurality of human pluripotent or human multipotent cells are attached to the matrix coating.
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Dependencies are inferred from the claim text.