Pdx1 expressing endoderm
AssigneeViaCyte, Inc.
InventorsKevin Alan D'Amour, Alan D. Agulnick, Susan Eliazer, Emmanuel E. Baetge
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Abstract
Disclosed herein are cell cultures comprising PDX1-positive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified PDX1-positive endoderm cells as well as methods for enriching, isolating and purifying PDX1-positive endoderm cells from other cell types. Methods of identifying differentiation factors capable of promoting the differentiation of endoderm cells, such as PDX1-positive foregut endoderm cells and PDX1-negative definitive endoderm cells, are also disclosed.
Claims21
1-18. (canceled)
An in vitro population of cells comprising PDX1-positive endoderm cells, wherein said population of cells is in contact with a retinoid.
An in vitro population of cells comprising PDX1-positive endoderm cells expressing a marker selected from the group consisting of HOXA13, PROX1 and HOXC6.
An in vitro population of PDX1-positive endoderm cells obtained by differentiation of pluripotent cells, wherein the population of PDX1-positive endoderm cells expresses HOXA13, PROX1 or HOXC6.
Dependencies are inferred from the claim text.