Large scale cell harvesting method for pack-bed culture device

A large scale cell harvesting method, comprising:

• providing a pack-bed culture device configured with a culture chamber having a cell growing space defined within the culture chamber, wherein
• the cell growing space is loosely packed with a plurality of matrix means, wherein the matrix means are porous and have porous matrixes therein,
• a plurality of cells grow on the porous matrixes, and
• a void volume is unoccupied space defined by the matrix means;
• in the cell growing space;
• performing a cell detaching step at least comprising shaking or spinning the culture chamber, wherein the void volume is large enough for the matrix means collide to each other or to the culture chamber or flush by liquid to generate momentum whereby the cells detach from the porous matrixes;
• and
• harvesting the cells.