High Efficiency and High Precision Microfluidic Devices and Methods

A microfluidic device comprising an array of fluidically interconnected unit cells, wherein each unit cell comprises:

• a) a first microfluidic flow path (250) in fluid communication with:
• 1) a reaction Chamber (400), and
• 2) a first microfluidic bus line (220), wherein said first microfluidic bus line is in fluidic communication with a sample source reservoir;
• b) a first valve (V1) situated in said first microfluidic flow path;
• c) a second valve (V2) situated in said first microfluidic flow path;
• d) a second microfluidic flow path (300) in fluidic communication with:
• 1) said first microfluidic flow path at a junction between said first and second valves, and
• 2) a second microfluidic bus line (230), wherein said second microfluidic bus line is in fluidic communication with a reagent source reservoir
• e) a third valve (V3) situated
• 1) in said second microfluidic flow path (300), or
• 2) in said second microfluidic bus line (230) positioned between the microfluidic flow channel of said unit cell and the second microfluidic flow path of an adjacent unit cell.