Thermostable enzymes and methods of making and using the same
AssigneeUniversity of Calcutta
InventorsArka Mukhopadhyay, Hirak Kumar Patra, Krishanu Chakraborti, Anjan Kr. Dasgupta, Dhrubajyoti Chattopadhyay
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Abstract
Provided herein are compositions and methods for enhancing enzyme activity, half-life and/or thermostability. Also provided herein are compositions and methods including the enhanced enzymes. Also provided herein are methods and compositions related to improved pectinolytic enzymes, such as pectate lyase, which exhibit enhanced activity, thermostability and/or longer half-life.
Claims25
A composition, comprising:
- at least one nanoparticle, comprising an apatite moiety;
- and
- at least one enzyme in contact with the nanoparticle, the apatite moiety, or both, wherein the enzyme comprises a pectinolytic enzyme.
(canceled)
-14. (canceled)
A method of making a thermostable enzyme composition, the method comprising:
- combining to form a mixture:
- a) a plurality of nanoparticles, wherein at least some of the nanoparticles comprise an apatite moiety;
- and
- b) at least one enzyme;
- under conditions in which the enzyme is in contact with the nanoparticles, the apatite moiety, or both.
-24. (canceled)
A method of treating a pectin-containing material, the method comprising:
- contacting the material with a composition comprising:
- a) at least one nanoparticle, comprising an apatite moiety;
- and
- b) a pectinolytic enzyme in contact with the nanoparticle, the apatite moiety, or both, for a time and under conditions wherein at least some of the pectin in the material is cleaved by the enzyme.
(canceled)
A kit comprising:
- a) an pectinolytic enzyme;
- b) a plurality of nanoparticles comprising an apatite moiety;
- and
- c) instructions for combining the enzyme and the nanoparticles to form an enzyme composition.
(canceled)
Dependencies are inferred from the claim text.