MRNA Interferase from Myxococcus Xanthus
AssigneeUniversity of Medicine and Dentistry of New Jersey
InventorsMasayori Inouye, Hirofumi Nariya
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Abstract
A regulated deployment of a toxin gene for developmental programmed cell death in bacteria is described. M. xanthus is demonstrated to have a solitary mazF gene that lacks a cotranscribed antitoxin gene. Deletion of mazF results in elimination of the obligatory cell death during development causing dramatic reduction in spore formation. Surprisingly, MrpC functions as a MazF antitoxin and a mazF transcription activator. Transcription of mrpC and mazF is negatively regulated via MrpC phosphorylation by a Ser/Thr kinase cascade. Various methods of exploiting this novel pathway are described herein.
Claims7
1-6. (canceled)
An isolated Myxococcus xanthus (mazF-mx) polypeptide.
(canceled)
10-11. (canceled)
A method of production of a polypeptide having endoribonuclease activity comprising:
- a. transforming a host cell by introducing a polynucleotide encoding maxF-mx into the host cell, and
- b. culturing the transformed host cell.
A promoter region of mazF-mx having the DNA sequence of SEQ ID NO:
- 14.
Dependencies are inferred from the claim text.