Cell-free system for converting methane into fuel and chemical compounds
AssigneeGreenLight Biosciences, Inc.
InventorsWilliam Jeremy Blake, James R. Swartz
Life of this patent
Estimating the term…
Abstract
The present disclosure relates, in some aspects, to cell-free methods and systems for large-scale conversion of methane to isobutanol, comprising combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture, and incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
Claims29
A cell-free method for large-scale conversion of methane to isobutanol, comprising:
- combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol, to form a cell-free reaction mixture;
- and
- incubating under suitable conditions the cell-free reaction to convert methane to isobutanol.
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A cell-free method for large-scale conversion of methane to pyruvate, comprising:
- combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to pyruvate, to form a cell-free reaction mixture;
- and
- incubating under suitable conditions the cell-free reaction to convert methane to pyruvate.
18-32. (canceled)
A cell-free method for large-scale conversion of methane to a biofuel or other chemical compound, comprising:
- combining, in a bioreactor at elevated pressure, methane, oxygen, and cell lysates containing methane monooxygenase, methanol dehydrogenase, enzymes that catalyze the conversion of formaldehyde to pyruvate, and enzymes that catalyze the conversion of pyruvate to a biofuel or other chemical compound, to form a cell-free reaction mixture;
- and
- incubating under suitable conditions the cell-free reaction to convert methane to the biofuel or other chemical compound.
34-37. (canceled)
39-48. (canceled)
A cell-free system for large-scale conversion of methane to isobutanol, the system comprising a bioreactor that comprises:
- a gas phase comprising methane and oxygen;
- and
- an aqueous phase comprising a cell lysate containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to isobutanol.
50-57. (canceled)
A cell-free system for large-scale conversion of methane to pyruvate, the system comprising a bioreactor that comprises:
- a gas phase comprising methane and oxygen;
- and
- an aqueous phase comprising a cell lysate containing methane monooxygenase, methanol dehydrogenase, and enzymes that catalyze the conversion of formaldehyde to pyruvate.
59-66. (canceled)
A cell-free system for large-scale conversion of methane to pyruvate, the system comprising a bioreactor that comprises:
- a gas phase comprising methane and oxygen;
- and
- an aqueous phase comprising a cell lysate containing methane monooxygenase, methanol dehydrogenase, enzymes that catalyze the conversion of formaldehyde to pyruvate, and enzymes that catalyze the conversion of pyruvate to a biofuel or other chemical compound, to form a cell-free reaction mixture.
68-75. (canceled)
Dependencies are inferred from the claim text.