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AssigneeInvista North America S.A R.L.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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This document describes biochemical pathways for producing one or more of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine and 1,7-heptanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl groups, in a C7 aliphatic backbone substrate produced from succinate semialdehyde or pyruvate. These pathways, metabolic engineering and cultivation strategies described herein rely on the aldol condensation of succinate semialdehyde and pyruvate.
A method for biosynthesizing a product selected from the group consisting of pimelic acid, 7-aminoheptanoate, 7-hydroxyheptanoate, heptamethylenediamine and 1,7-heptanediol, said method comprising enzymatically synthesizing a seven carbon chain aliphatic backbone from succinate semialdehyde and pyruvate via aldol condensation and enzymatically forming two terminal functional groups selected from the group consisting of carboxyl, amine, and hydroxyl groups in said backbone, thereby forming the product.
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A recombinant host comprising at least one exogenous nucleic acid encoding an 4-hydroxy-2-oxopimelate aldolase, a 2-hydroxyhepta-2,4-dienedioate hydratase, an enoate reductase, a 2-hydroxyglutarate dehydrogenase, a glutaconate CoA-transferase, a 2-hydroxyglutaryl-CoA dehydratase, and an enoyl-CoA reductase or an enoyl-[acp] reductase, said host producing pimeloyl-CoA.
A recombinant host comprising at least one exogenous nucleic acid encoding an 4-hydroxy-2-oxopimelate aldolase, a 2-hydroxyhepta-2,4-dienedioate hydratase, an enoate reductase, a 2-hydroxyglutarate dehydrogenase, a glutaconate CoA-transferase, a 2-hydroxyglutaryl-CoA dehydratase, and a carboxylate reductase, said host producing pimelate semialdehyde.
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Dependencies are inferred from the claim text.