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AssigneeINVISTA North America S.a r.I.
InventorsAdriana Leonora Botes, Alex Van Eck Conradie, Changlin Chen, Paul S. Pearlman
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This document describes biochemical pathways for producing pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine or 1,7-heptanediol by forming two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C7 aliphatic backbone substrate. These pathways, metabolic engineering and cultivation strategies described herein rely on the carbon chain elongation enzymes or homologs thereof associated with the cyclohexane carboxylate biosynthesis from Syntrophus aciditrophicus or 2-aminoadipate lysine biosynthesis.
A method for biosynthesizing a product selected from the group consisting of pimelic acid, 7-aminoheptanoic acid, 7-hydroxyheptanoic acid, heptamethylenediamine, and 1,7-heptanediol, said method comprising enzymatically synthesizing a seven carbon chain aliphatic backbone using enzymes associated with cyclohexane carboxylate biosynthesis or the 2-aminoadipate lysine biosynthesis pathway, and enzymatically forming one or two terminal functional groups selected from the group consisting of carboxyl, amine, and hydroxyl groups in said backbone, thereby forming the product.
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A recombinant host comprising at least one exogenous nucleic acid encoding i) a β-ketothiolase or a β-ketoacyl[acp] synthase, (ii) a 6-oxo-cyclohex-1-ene-carbonyl-CoA hydrolase or an enoyl-CoA hydratase, and (iii) a trans-2-enoyl-CoA reductase, an enoyl[acp] reductase, or a 2-ketocyclohexanecarboxyl-CoA hydrolase, said host producing pimeloyl-CoA.
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Dependencies are inferred from the claim text.