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AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsMichael Wayne Graham, Robert Norman Rice, Peter Michael Waterhouse
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The present invention relates generally to a method of modifying gene expression and to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention utilizes recombinant DNA technology to post-transcriptionally modify or modulate the expression of a target gene in a cell, tissue organ or whole organism, thereby producing novel phenotypes. Novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or target gene in an organism when introduced thereto are also provided.
1-43. (canceled)
A double-stranded DNA construct which is capable of delaying, repressing, or otherwise reducing the expression of a target gene, wherein the double-stranded DNA construct comprises a foreign nucleic acid molecule comprising multiple copies of a nucleotide sequence that is more than 20 nucleotides long and which is substantially identical to a region of the target gene, and wherein the foreign nucleic acid molecule is placed operably under the control of both a first promoter sequence and a second promoter sequence which are each operable in a cell, wherein said promoters are located at the distal and proximal ends of the foreign nucleic acid molecule.
A prokaryotic cell comprising the double-stranded DNA construct of claim 12.
Dependencies are inferred from the claim text.