Methods and compositions for determining the purity of chemically synthesized nucleic acids
AssigneesVeri-Q, Inc., North Carolina State University
InventorsPaul F. Agris, Christopher D.J. Pearce, Lloyd G. Mitchell
Life of this patent
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Abstract
This application describes an antibody that specifically binds to a synthetic oligomer (e.g., an oligonucleotide or oligopeptide) having a organic protecting group covalently bound thereto, which antibody does not bind to that synthetic oligomer when the organic protecting group is not covalently bound thereto. Methods of making and using such antibodies are also disclosed, along with cells for making such antibodies and articles carrying immobilized oligomers that can be used in assay procedures with such antibodies.
Claims9
1-25. (canceled)
A method of making an antibody that specifically binds to a synthetic oligonucleotide having a organic protecting group covalently bound thereto, which antibody does not bind to the said synthetic oligonucleotide when said organic protecting group is not covalently bound thereto, said method comprising the steps of:
- synthesizing said synthetic oligonucleotide on a solid particulate support with said organic protecting group covalently bound to said synthetic oligonucleotide, or synthesizing a nucleotide on said solid support with said organic protecting group bound to said nucleotide;
- and then, without removing said oligonucleotide or nucleotide from said solid support;
- immunizing an animal with said synthetic oligonucleotide or nucleotide bound to said solid support in an amount sufficient to produce said antibody.
33-55. (canceled)
Dependencies are inferred from the claim text.