US 2014/0193854 A1 · Method for Mass-Producing Antifreeze Protein Derived from Polar Yeast

Abstract

The present invention relates to a method for mass-producing an antifreeze protein derived from a polar yeast, and more particularly, to a method for mass-producing an antifreeze protein derived from Leucosporidium sp., which is the polar yeast, for synthesizing a recombinant polynucleotide by optimizing and altering a gene, which codes the antifreeze protein derived from the polar yeast, for a yeast expression system, and for expressing same using the yeast expression system.

Claims4

Dependent claims

A method for mass-producing an antifreeze protein derived from arctic yeast or a culture medium containing the same, the method comprising the steps of:

(1) synthesizing a polynucleotide comprising the nucleotide sequence of SEQ. ID. NO:
1;
(2) introducing the polynucleotide of step (1) into a Pichia pastoris-derived expression vector;
(3) culturing a yeast transformant transformed with the recombinant expression vector;
and
(4) obtaining a culture medium from step (3).

3 more elements

Dependencies are inferred from the claim text.

Method for Mass-Producing Antifreeze Protein Derived from Polar Yeast

Life of this patent

Method for Mass-Producing Antifreeze Protein Derived from Polar Yeast

Life of this patent

Abstract

Claims4