Adipose tissue collection and pre-processing devices for use in liposuction procedure

A method for processing an input composition comprising a tissue sample composition and one or more liquid phase compositions to extract cells of interest, comprising the steps of:

• engaging a first automated apparatus for:
• shredding at least a portion of the input composition;
• and
• separating said portion of the input composition into a fibrous composition that is relatively rich in a fibrous tissue component and an intermediate cell product composition that is relatively rich in the cells of interest;
• and
• engaging a second automated apparatus to separate the one or more liquid phases from the fibrous composition or the intermediate cell product composition, or both.

A tissue processing device for extracting cells of interest from a first input composition comprising a tissue sample composition and one or more liquid phase compositions, comprising:

• a container comprising a first compartment and a second compartment;
• first separation means within the first compartment for separating at least a portion of the input composition into a first solid composition that is relatively rich in the tissue sample composition, and a first liquid composition that is relatively rich in the one or more liquid phase compositions;
• a gap between the first compartment and the second compartment;
• means within the gap for shredding at least a portion of the first solid composition, and for separating said portion of the first solid composition into a fibrous composition and an intermediate cell product composition so as to transfer at least a portion of the intermediate cell product composition to the second compartment while retaining the majority of the fibrous composition outside the second compartment, wherein the fibrous composition is relatively rich in a fibrous tissue component and the intermediate cell product composition is relatively rich in the cells of interest.

A method for processing an input composition comprising a tissue sample composition and one or more liquid phase compositions to extract cells of interest, comprising the steps of:

• injecting the input composition into the first compartment of a container comprising:
• a first compartment and a second compartment;
• a gap between the first compartment and the second compartment;
• and
• a first plurality of blades within the gap;
• separating at least a portion of the input composition into a first solid composition that is relatively rich in the tissue sample composition, and a first liquid composition that is relatively rich in the one or more liquid phase compositions;
• sweeping the first plurality of blades against a second plurality of blades in contact with the portion of the first solid composition, wherein the second plurality of blades is configured to be in an offset tooth pattern with the first plurality of blades, and wherein the openings defined by the spaces between the offset teeth are large enough to permit passage of at least a portion of the first solid composition, and small enough to prevent passage of the majority of the fibrous composition after the first plurality of blades have swept against the second plurality of blades;
• and
• separating said portion of the first solid composition into a fibrous composition and an intermediate cell product composition so as to transfer at least a portion of the intermediate cell product composition to the second compartment while retaining the majority of the fibrous composition outside the second compartment, wherein the fibrous composition is relatively rich in a fibrous tissue component and the intermediate cell product composition is relatively rich in the cells of interest.

A tissue processing device for extracting cells of interest from a tissue sample composition, comprising:

• a digestion chamber comprising a first section and a second section, the first section and the second section separated by a first filter, wherein the first filter is configured to allow passage of particles of a particular size or smaller, while retaining particles of the particular size or larger within the first section of the digestion chamber, wherein the particular size is larger than the cells of interest;
• agitation means for agitating a composition within the digestion chamber;
• a piston configured within the first section so as to be capable of displacing fluids in either of two directions across the first filter;
• and
• a system of one or more valves configurable in a first configuration to allow fluid communication between the second section and a waste container, configurable in a second configuration to allow fluid communication between the second section and a suspension medium container, and configurable in a third configuration to allow fluid communication with the inlet of a cell concentrator.

A method for processing a tissue sample composition to extract cells of interest, comprising the steps of:

• introducing the tissue sample composition into a digestion chamber comprising a first section and a second section, the first section and the second section separated by a first filter, wherein the first filter is configured to allow passage of particles of a particular size or smaller, while retaining particles of the particular size or larger within the first section of the digestion chamber, wherein the particular size is larger than the cells of interest;
• introducing a suspension medium into the first section of the digestion chamber;
• allowing the contents of the first section of the digestion chamber to digest for a predetermined length of time, to create a digested cell composition;
• pushing or suctioning at least a portion of the digested cell composition through the first filter;
• passing said portion of the digested cell composition through a passage toward the inlet of a cell concentrator;
• and
• passing at least a part of said portion of the digested cell composition through the cell concentrator.