Real-time detection of influenza virus

A method of performing a trend analysis on the concentration of an analyte indicative of an influenza viral infection in a subject, comprising:

• a) providing a fluidic device comprising at least one sample collection unit and an immunoassay assembly containing immunoassay reagents;
• b) actuating said fluidic device and directing said immunoassay reagents within said fluidic device;
• c) allowing a sample of bodily fluid obtained from said subject to react with said immunoassay reagents contained within said assay immunoassay assembly to yield a detectable signal indicative of the presence of said analyte in said sample;
• d) detecting said detectable signal generated from said analyte collected in said sample of bodily fluid;
• and
• e) repeating steps a) through d) for one or more further bodily fluid samples obtained from said subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis.

A method of performing a trend analysis on the concentration of influenza viral particles in each of a plurality of bodily fluid samples obtained from a subject suspected of containing influenza viral particles, comprising:

• a) providing an immunoassay assembly, wherein the assembly comprises a first antibody that binds specifically to a first antigen, wherein the first antibody is bound to a surface of the assembly;
• b) reacting a bodily fluid sample obtained from said subject in the immunoassay assembly in the presence of immunoassay reagents comprising a second antibody that binds specifically to a second antigen which is distinct from the first antigen, wherein the second antibody is labeled with a detectable label, wherein either the first antigen comprises a hemagglutinin and the second antigen comprises a neuraminidase, or the first antigen comprises a neuraminidase and the second antigen comprises a hemagglutinin, and wherein said reaction yields an immune complex comprising the first antibody bound to the first antigen, and the second antibody bound to the second antigen;
• c) washing the assembly to remove excess immunoassay reagents;
• d) detecting a signal generated by the detectable label from the immune complex, thereby indicating the presence of the particle;
• and
• e) repeating steps a) through d) for one or more further bodily fluid samples obtained from the subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis on the concentration of an analyte indicative of an influenza viral infection in a subject.

A method of detecting a trend in the concentration of influenza viral particles in a plurality of bodily fluid samples obtained from a subject, comprising:

• a) allowing a bodily fluid sample suspected of containing an influenza viral particle to react with a first immunoassay reagent and a second immunoassay reagent, both of which immunoassay reagents are contained within an immunoassay assembly of a cartridge, wherein said first immunoassay reagent binds to a hemagglutinin molecule to form a first immune complex on said influenza viral particle, and wherein said second immunoassay reagent binds to a neuraminidase molecule to form a second immune complex on said influenza viral particle, said immune complexes yielding one or more detectable signals that indicate the simultaneous presence of hemagglutinin and neuraminidase on said influenza viral particle wherein either the first or second immunoassay reagent is immobilized on a solid support;
• b) detecting said one or more detectable signals generated from said immune complexes, wherein said simultaneous presence of hemagglutinin and neuraminidase is indicative of the presence of and concentrations of said influenza viral particles in the sample;
• and
• c) repeating steps a) and b) for one or more further bodily fluid samples obtained from the subject over a period of time to detect concentrations of said analyte over said period of time, thereby performing said trend analysis.