Formulation of Curcuminoids with Enhanced Bioavailability of Curcumin, Demethoxycurcumin, Bisdemethoxycurcumin and method of preparation and uses thereof

A method of preparing a curcuminoid mixture, the curcuminoid mixture comprising curcumin, demethoxycurcumin and bisdemethoxycurcumin in a weight ratio of curcumin:demethoxycurcumin:bisdemethoxycurcumin ranging from about 0.8:1:1 to about 1:1.1:1.2, the method comprising:

• drying a rhizome of turmeric to form a dried turmeric rhizome;
• powdering the dried turmeric rhizome to form a dried turmeric rhizome powder;
• adding ethyl acetate to the dried turmeric rhizome powder to obtain an ethyl acetate extract solution and a residue;
• separating the residue from the ethyl acetate extract solution;
• stripping solvent from the filtered ethyl acetate extract solution to form an ethyl acetate extract;
• cooling the ethyl acetate extract to obtain crystals of curcuminoid and a liquid comprising essential oil of turmeric and curcuminoids that did not crystallize;
• steam distilling the liquid comprising essential oil of turmeric and curcuminoids that did not crystallize to obtain essential oil of turmeric and a waste residue 2;
• loading the waste residue 2 onto a silica column;
• washing the silica column with methanol and collecting three fractions of methanol eluate, wherein the three fractions are Fraction 1, Fraction 2 and Fraction 3;
• combining Fraction 2 and Fraction 3 to obtain a combined methanol fraction;
• concentrating the combined methanol fraction under heat and vacuum to obtain a concentrated methanol fraction;
• drying the concentrated methanol fraction to obtain a dried methanol extract;
• powdering the dried methanol extract to obtain a powder of the curcuminoid mixture comprising curcumin, demethoxycurcumin and bisdemethoxycurcumin in a weight ratio of curcumin:demethoxycurcumin:bisdemethoxycurcumin of about 0.8:1:1 to about 1:1.1:1.2.

A method of preparing a composition for enhanced bioavailability of curcumin, demethoxycurcumin and bisdemethoxycurcumin, the composition comprising a curcuminoid mixture and essential oil of turmeric, the method comprising:

• drying a rhizome of turmeric to form a dried turmeric rhizome;
• powdering the dried turmeric rhizome to form a dried turmeric rhizome powder;
• adding ethyl acetate to the dried turmeric rhizome powder to obtain an ethyl acetate extract solution and a residue;
• separating the residue from the ethyl acetate extract solution;
• stripping solvent from the filtered ethyl acetate extract solution to form an ethyl acetate extract;
• cooling the ethyl acetate extract to obtain crystals of curcuminoid and a liquid comprising essential oil of turmeric and curcuminoids that did not crystallize;
• steam distilling the liquid comprising essential oil of turmeric and curcuminoids that did not crystallize to obtain essential oil of turmeric and a waste residue 2;
• loading the waste residue 2 onto a silica column;
• washing the silica column with methanol and collecting three fractions of methanol eluate, wherein the three fractions are Fraction 1, Fraction 2 and Fraction 3;
• combining Fraction 2 and Fraction 3 to obtain a combined methanol fraction;
• concentrating the combined methanol fraction under heat and vacuum to obtain a concentrated methanol fraction;
• drying the concentrated methanol fraction to obtain a dried methanol extract;
• powdering the dried methanol extract to obtain a powder of the curcuminoid mixture, the curcuminoid mixture comprising curcumin, demethoxycurcumin and bisdemethoxycurcumin in a weight ratio of curcumin:demethoxycurcumin:bisdemethoxycurcumin of about 0.8:1:1 to about 1:1.1:1.2;
• suspending the powder of the curcuminoid mixture in water to form a suspension;
• adding essential of turmeric to the suspension to obtain a mixture;
• homogenizing the mixture to obtain a slurry;
• drying the slurry under heat and vacuum to form a uniform blend of the composition for enhanced bioavailability of curcumin, demethoxycurcumin and bisdemethoxycurcumin, wherein the weight ratio of curcumin:demethoxycurcumin:bisdemethoxycurcumin ranges from about 0.8:1:1 to about 1:1.1:1.2, and wherein the essential oil of turmeric comprises 4.5% Ar-turmerone.