Collagen based materials and uses related thereto
AssigneesEmory University, Beth Israel Deaconess Medical Center, Inc.
InventorsElliot Chaikof, Jeffrey Caves, Vivek Ashok Kumar, Adam W. Martinez
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Abstract
This disclosure relates to materials fabricated from collagen and uses relates thereto. Typically, layers of collagen are stretched during a curing period and optionally coated or impregnated with an elastin like protein. In certain embodiments, these materials can be used in tissue repair or arranged into cylinders and utilized as a prosthetic vascular graft.
Claims34
A synthetic material comprising fibril collagen matrix with a collagen density of greater than 600 micrograms per square centimeter and the collagen fibers maintain D-periodicity.
A method of making a sheet of collagen comprising
- a) mixing an acid solution comprising acid soluble collagen with a buffer under conditions such that a collagen gel forms;
- b) incubating the collagen gel in an aqueous buffer solution at a neutral pH for more than one day providing a cured layer of collagen;
- c) separating the cured layer of collagen from the buffer solution;
- and
- d) drying the cured layer of collagen to provide dried collagen.
A method of producing a material comprising a layer of collagen and a layer of elastic polymer comprising
- a) cooling an acid solution to less than 15 degrees Celsius providing a cooled solution comprising,
- acid soluble collagen, and
- a protein comprising peptide repeats of [YaaPUaaXaaZaap]n (SEQ ID NO:1)
- wherein Yaa is glycine, alanine, lucine, isolucine, or valine;
- P is Pro;
- Uaa is glycine, alanine, lucine, isolucine, or valine;
- Xaa is aspartic acid, glutamic acid, glycine, alanine, lucine, isolucine, or valine or any amino acid except Pro;
- Zaa is glycine, alanine, lucine, isolucine, or valine;
- p is 0, 1, 2, 3, 4, 5, or 6;
- and
- n is 1 to 1000;
- b) neutralizing the cooled solution such that a collagen layer forms;
- and
- c) warming the solution under conditions such that an elastic layer forms.
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A method of producing a material comprising
- a) contacting a dried collagen sheet with a solution cooled to less than 15 degree Celsius wherein the cooled solution comprises a protein comprising peptide repeats of [YaaPUaaXaaZaap]n (SEQ ID NO:1) wherein
- Yaa is glycine, alanine, lucine, isolucine, or valine;
- P is Pro;
- Uaa is glycine, alanine, lucine, isolucine, or valine;
- Xaa is aspartic acid, glutamic acid, glycine, alanine, lucine, isolucine, or valine or any amino acid except Pro;
- Zaa is glycine, alanine, lucine, isolucine, or valine;
- p is 0, 1, 2, 3, 4, 5, or 6;
- and
- n is 1 to 1000;
- and
- b) warming the solution under conditions such that an elastic polymer layer forms over the dried collagen sheet.
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Dependencies are inferred from the claim text.