Methods for preparation of neo-cartilage constructs
AssigneeHistogenics Corporation
InventorsLaurence J. B. Tarrant, Sonya Shortkroff, Shuichi Mizuno, Akihiko Kusanagi, Toshimasa Tokuno, Robert Lane Smith
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Abstract
The invention generally relates to systems (i.e. constructs) for repairing cartilage and methods for preparing the same that introduce a bioactive agent into a culture medium, suspension, scaffold, solution incorporated into the pores of the scaffold, or combinations thereof. The introduction of a bioactive agent promotes production of neo-cartilage (i.e. immature hyaline cartilage) in the system, both ex-vivo and in-vivo.
Claims28
A system for repairing cartilage, the system comprising
- an acellular collagen matrix comprising a plurality of pores, and
- a solution disposed within the pores, said solution being treated with at least one bone inducing agent,
- wherein said acellular matrix comprising the solution is lyophilized.
A method of repairing cartilage in a subject, said method comprising the steps of:
- providing an acellular collagen matrix comprising a plurality of pores,
- soaking said matrix in a solution to dispose the solution within said plurality of pores, wherein the solution was treated with at least one bone inducing agent prior to soaking;
- lyophilizing the acellular matrix comprising the solution;
- and
- implanting the lyophilized acellular collagen matrix into a cartilage lesion in said subject.
Dependencies are inferred from the claim text.