Biodegradable high-efficiency dengue vaccine, method for making the same, and pharmaceutical composition comprising the same

A method for making a biodegradable high-efficiency dengue vaccine, comprising the steps of:

• preparing a first solution comprising a first biodegradable macromolecule with a first electric property;
• dissolving a dengue viral protein with the same electric property as the first biodegradable macromolecule into the first solution to be a mixture solution with the first electric property;
• preparing a second solution comprising a second biodegradable macromolecule with a second electric property, wherein the first electric property is opposite to the second electric property;
• and
• adding the mixture solution into the second solution for forming a biodegradable nanocomplex by attraction force between the different electric properties, wherein the dengue viral protein is held in the biodegradable nanocomplex.