Stable and soluble antibodies inhibiting tnf alpha
AssigneeESBA Tech, an Alcon Biomedical Research Unit LLC
InventorsLeonardo Borras, Tea Gunde, David Urech
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Abstract
The present invention relates to particularly stable and soluble scFv antibodies and Fab fragments specific for TNF, which comprise specific light chain and heavy chain sequences that are optimized for stability, solubility, in vitro and in vivo binding of TNF, and low immunogenicity. Said antibodies are designed for the diagnosis and/or treatment of TNF-mediated disorders. The nucleic acids, vectors and host cells for expression of the recombinant antibodies of the invention, methods for isolating them and the use of said antibodies in medicine are also disclosed.
Claims29
An immunobinder which specifically binds human TNFα, the immunobinder comprising:
- (a) a human heavy chain variable framework sequence and CDR H1, CDR H2 and CDR H3 sequences stemming from a rabbit immunobinder, wherein the human heavy chain variable region framework has at least 90% identity to SEQ ID NO:1;
- and
- (b) a human light chain variable framework sequence and CDR L1, CDR L2 and CDR L3 sequences stemming from a rabbit immunobinder, wherein the human light chain variable region framework sequence has at least 85% identity to SEQ ID NO:2.
Dependencies are inferred from the claim text.