Method for enzymatic cross-linking of a protein
AssigneeLifebond Ltd.
InventorsOrahn PREISS-BLOOM, Guy TOMER, Nadav BRAMSON
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Abstract
A method for cross-linking albumin for use as a sealant or glue for a biological system, for example to induce hemostasis and/or prevent leakage of any other fluid from a biological tube or tissue, such as lymph for example. The cross-linked albumin may optionally and preferably be applied as part of a bandage for example. In other embodiments, the present invention provides a method of enzymatically cross-linking globular proteins, by altering the structure of the protein to improve the accessibility of the protein to the cross-linking enzyme.
Claims36
A gel composition comprising denatured albumin, transglutaminase, a chaotropic agent and a disulfide bond reducing agent in a pH range of from 6 to 9.
A method of preparation of a gel composition, comprising:
- providing albumin with a pH value of from 4 to 6;
- heat denaturing said albumin to form denatured precipitated albumin;
- dissolving said denatured precipitated albumin with a disulfide bond reducing agent at a pH value of no more than 3 or of at least 9 to form reduced soluble albumin;
- adjusting a pH value of said reduced albumin to be between 6 and 9;
- and combining transglutaminase with said reduced albumin to form a combination.
A gel composition comprising heat denatured albumin, transglutaminase, a chaotropic agent and a disulfide bond reducing agent in a pH range of from 6 to 9.
A method of preparation of a gel composition, comprising:
- providing albumin with a pH value of from 6 to 9;
- denaturing said albumin with a chaotropic agent to form denatured albumin;
- treating said denatured albumin with a disulfide bond reducing agent at a pH value of 6 to 9 to form reduced albumin;
- and combining transglutaminase with said reduced albumin to form a combination.
Dependencies are inferred from the claim text.