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US 2014/0193382 A1Published application
Endogenous Expression of HLA-G and/or HLA-E by Mesenchymal Cells
AssigneeEscape Therapeutics, Inc.
InventorBasil M. HANTASH
Life of this patent
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Abstract
Methods and compositions are provided for the identification and isolation of mammalian HLA-G+ MSC, HLA-E+ MSC, or HLA-G+/HLA-E+ MSC. The methods of the invention provide a means to obtain enriched HLA-G+ MSC, HLA-E+ MSC, or HLA-G+/HLA-E+ MSC populations.
Claims8
1-15. (canceled)
A method of cell transplantation into a human recipient, the method comprising:
- (a) providing an isolated composition of human cells comprising an enriched population of human adult adipose tissue derived mesenchymal stem cells or bone marrow-derived mesenchymal stem cells, wherein at least 90% of said mesenchymal stem cells express cell surface HLA-G, HLA-E, or both HLA-G and HLA-E, and upon initial isolation from adipose tissue or bone marrow tissue, maintain cell surface expression of HLA-G, HLA-E, or both HLA-G and HLA-E, after at least two passages in non-differentiating in vitro culture conditions;
- and
- (b) injecting the isolated composition of step (a) into a human recipient, wherein the cells of the isolated composition of step (a) are allogeneic or autologous with respect to the human recipient, and wherein a perfect match of histocompatibility antigens is not required between the isolated composition of human cells of step (a) and the human recipient.
Dependencies are inferred from the claim text.