Mesenchymal Stromal Cell Populations and Methods of Making Same
AssigneeAastrom Biosciences, Inc.
InventorsFrank Zeigler, Ronnda L. Bartel
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Abstract
The present invention provides compositions of mesenchymal stromal cells which express B7-H3, their subsequent use in tissue repair, improved methods of producing tissue repair cells and method of producing a substantially pure population of CD14+ autofluorescent macrophages.
Claims32
An isolated mesenchymal stromal cell composition wherein the mesenchymal stromal cells express B7 homolog 3 (B7-H3).
An isolated cell composition comprising mesenchymal stromal cells that express B7 homolog 3 (B7-H3).
A mesenchymal stromal cell that has been genetically engineered to stably express B7 homolog 3 (B7-H3) on the surface of the cell.
A method of producing a cell composition comprising a mixed population of cells of hematopoietic, mesenchymal and endothelial lineage, wherein the cell composition is characterized as containing 5-75% viable CD90+ cells with the remaining cells in said composition being CD45+, CD31+, CD14+, and auto+, comprising culturing mononuclear cells in the presence of
- i) B7-H3 polypeptide,
- ii) a V-set and Ig domain-containing 4 (VSIG4) polypeptide, or
- iii) both i) and ii).
A method of producing a substantially pure population of CD14+ autofluorescent macrophages comprising culturing mononuclear cells in the presence of
- i) a B7-H3 polypeptide,
- ii) a V-set and Ig domain-containing 4 (VSIG4) polypeptide, or
- iii) both i) and ii);
- and isolating said CD14+ autofluorescent macrophages from said culture.
Dependencies are inferred from the claim text.