Methods for establishing and improving the survival of a population of pancreatic progenitor or stem cells
AssigneesCity of Hope, California Institute of Technology
InventorHsun Ku
Life of this patent
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Abstract
In some embodiments, methods for improving the survival of pancreatic β-cell progenitors in culture are provided. Such methods may include contacting a population of pancreatic progenitor cells with an amino acid (aa) sequence comprising IKVAV (SEQ ID NO:1). In other embodiments, methods for (i) verifying the establishment of a population of pancreatic progenitor or stem cells and (ii) methods for generating or establishing a population of pancreatic endocrine progenitor cells in vitro are provided.
Claims20
A method of improving the survival of pancreatic β-cell progenitors in culture comprising contacting a population of pancreatic progenitor cells with an amino acid sequence comprising IKVAV (SEQ ID NO:1), wherein the amino acid sequence improves survival of the population of pancreatic β-cell progenitors.
A method of verifying the establishment of a population of pancreatic progenitor or stem cells comprising:
- isolating a population of cells from a pancreatic tissue sample that express CD133 and Sox9 (CD133+/Sox9+ cells);
- culturing at least one of the isolated CD133+/Sox9+ cells with an effective amount of R-Spondin 1 to establish at least one single-cell derived colony;
- and
- verifying that the single-cell derived colony comprises a population of pancreatic progenitor or stem cells by detecting expression of one or more pancreatic progenitor cell markers in one or more colony cells.
A method of establishing a population of pancreatic endocrine progenitor cells comprising
- isolating a population of cells from a pancreatic tissue sample that express CD133 and Sox9 (CD133+/Sox9+ cells);
- culturing at least one of the isolated CD133+/Sox9+ cells with an effective amount of R-Spondin 1 to establish at least one single-cell derived dense colony;
- dissociating at least one single-cell derived dense colony to form a single-cell suspension;
- culturing the single-cell suspension with an amino acid sequence comprising IKVAV (SEQ ID NO:1) in the absence of R-Spondin 1 to establish the population of pancreatic endocrine progenitor cells.
Dependencies are inferred from the claim text.