Hydrogel comprising catechol group-coupled chitosan or polyamine and poloxamer comprising thiol group coupled to end thereof, preparation method thereof, and hemostat using same

A method for preparing a hydrogel, the method comprising:

• (A) preparing a catechol group-coupled chitosan or polyamine;
• (B) preparing a polaxamer thiol group-coupled to the end thereof;
• (C) preparing a catechol-chitosan or catechol-polyamine solution by dissolving the catechol group-coupled chitosan or polyamine in a buffer;
• (D) preparing a thiol-polaxamer solution by dissolving the polaxamer thiol group-coupled in a buffer;
• (E) preparing an adhesive hydrogel solution by mixing the catechol-chitosan or catechol-polyamine solution of step (C) with the thiol-polaxamer solution of step (D);
• and
• (F) gelating the adhesive hydrogel solution of step (E).