Flow Cells For Differential Refractive Index Detection
AssigneeWaters Technologies Corporation
InventorAnthony C. Jeannotte
Life of this patent
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Abstract
A flow cell for differential refractive index detection. The flow cell includes a transparent body that extends from a first end to a second end along a longitudinal axis. The transparent body defines a sample prism chamber and reference prism chamber. The sample prism chamber is configured to allow fluid flow between the first and second ends of the transparent body along the longitudinal axis. The reference prism chamber is configured to receive a reference fluid. The sample and reference prism chambers each include a grating comprising a plurality of grooves extending along the longitudinal axis in the direction of fluid flow.
Claims30
A flow cell for differential refractive index detection, the flow cell comprising:
- a transparent body extending from a first end to a second end along a longitudinal axis, the transparent body defining:
- a sample prism chamber configured to allow fluid flow between the first and second ends of the transparent body along the longitudinal axis;
- and
- a reference prism chamber configured to receive a reference fluid;
- wherein the sample and reference prism chambers each comprise a grating comprising a plurality of grooves extending along the longitudinal axis in the direction of fluid flow.
A flow cell for differential refractive index detection, the flow cell comprising:
- a transparent body extending from a first end to a second end along a longitudinal axis, the transparent body defining:
- a first prism chamber configured to allow fluid flow between the first and second ends of the transparent body along the longitudinal axis;
- and
- a second prism chamber;
- wherein the first prism chamber comprises a grating comprising a plurality of grooves extending along the longitudinal axis in the direction of fluid flow.
A method comprising:
- directing an optical beam through a flow cell containing sample and reference prism chamber,
- conveying a fluid flow through the sample prism chamber;
- detecting presence of an analyte in the fluid flow based on a detected deflection of the optical beam,
- wherein the sample prism chamber includes a grating comprising a plurality of grooves extending parallel to the direction of the fluid flow.
A method comprising:
- molding an optical grade transparent polymer to form at lest part of a transparent body for a flow cell, the transparent body defining:
- a sample prism chamber configured to allow fluid flow through the transparent body and comprising a grating;
- and
- a reference prism chamber configured to receive a reference fluid;
- and
- depositing a protective surface layer on surfaces of the transparent body to make the sample and reference chambers resistant to fluids that are to be contained within sample and reference chambers.
Dependencies are inferred from the claim text.