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US 2014/0190903 A1Published application
System and method for particle filtration
AssigneeCytovera Inc.
InventorLotien R. Huang
Life of this patent
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Abstract
Embodiments of the present disclosure feature a filtration system comprising a filtration module for particle filtration and methods of using the device for the isolation of particles (e.g., viable cells). Advantageously, embodiments of the device provide for the high throughput filtration of large volumes of sample while preserving cell viability and providing high yields.
Claims49
A filtration device including at least one filtration unit, the at least one filtration unit comprising:
- a first sidewall;
- a second sidewall;
- a first fluid inlet defined between the first sidewall and the second sidewall, the first fluid inlet configured to receive a feed comprising a particle population and a fluid;
- a first row of pillars;
- a first plurality of pores defined by spacings between adjacent pillars in the first row of pillars, each of the first plurality of pores having an effective pore size smaller than a first physical pore size, the first physical pore size defined by the spacings between the adjacent pillars in the first row of pillars;
- a first flow chamber defined between the first row of pillars and the second sidewall and including a first flow chamber inlet and a first retentate outlet, the first flow chamber configured to direct a first retentate including at least a portion of the particle population to the first retentate outlet;
- a second flow chamber defined between the first row of pillars and the first sidewall and including a first filtrate outlet, the second flow chamber configured to direct a first filtrate including at least a portion of the fluid to the first filtrate outlet;
- wherein a spacing of pillars in the first row of pillars from the first sidewall that are located proximate the first flow chamber inlet being smaller than a spacing of pillars in the first row of pillars from the first sidewall located proximate the first retentate outlet;
- a second row of pillars;
- and
- a second plurality of pores defined by spacings between adjacent pillars in the second row of pillars, each of the second plurality of pores having an effective pore size smaller than a second physical pore size, the second physical pore size defined by the spacings between the adjacent pillars in the second row of pillars.
8 more elements
A filtration device including at least one filtration unit, the at least one filtration unit comprising:
- a first sidewall;
- a second sidewall;
- a first fluid inlet defined between the first sidewall and the second sidewall, the first fluid inlet configured to receive a feed comprising a particle population and a fluid;
- a first row of pillars including an upstream end and a downstream end;
- a first plurality of pores defined by spacings between adjacent pillars in the first row of pillars, each of the first plurality of pores having an effective pore size smaller than a first physical pore size, the first physical pore size defined by the spacings between the adjacent pillars in the first row of pillars;
- wherein a spacing of pillars in the first row of pillars from the first sidewall located proximate the upstream end of the first row of pillars is smaller than a spacing of pillars in the first row of pillars from the first sidewall located proximate the downstream end of the first row of pillars;
- a second row of pillars disposed downstream of the upstream end of the first row of pillars;
- and
- a second plurality of pores defined by spacings between adjacent pillars in the second row of pillars, each of the second plurality of pores having an effective pore size smaller than a second physical pore size, the second physical pore size defined by the spacings between the adjacent pillars in the second row of pillars;
- wherein a spacing of pillars in the second row of pillars from the second sidewall located proximate the upstream end of the second row of pillars is smaller than a spacing of pillars in the second row of pillars from the second sidewall located proximate the downstream end of the second row of pillars.
7 more elements
A filtration system comprising:
- a fluid line configured to receive a liquid suspension of cells comprising a target cell population suspended in a fluid;
- a first collection chamber;
- a second collection chamber;
- and
- at least one cartridge comprising
- an cartridge inlet,
- a first cartridge outlet fluidicly connected to the first collection chamber,
- a second cartridge outlet fluidicly connected to the second collection chamber, and
- at least one filtration unit, the at least one filtration unit having
- a filtration unit inlet fluidicly connected to the cartridge inlet,
- a first filtration unit outlet fluidicly connected to the first cartridge outlet,
- a second filtration unit outlet fluidicly connected to the second cartridge outlet,
- a flow chamber configured to separate the target cell population from at least a portion of the fluid by flow conditions, to direct the target cell population to the first filtration unit outlet, and to direct the at least a portion of the fluid to the second filtration unit outlet,
- the first collection chamber, the second collection chamber, and the at least one cartridge forming a closed system.
11 more elements
A method for cell processing comprising:
- introducing a feed sample comprising a viable target cell population and a nonviable cell population suspended in a fluid to a closed filtration system including a plurality of filtration units, each filtration unit including a flow chamber, a first outlet and a second outlet, the first outlet fluidicly connected to a first collection chamber and the second outlet fluidicly connected to a second collection chamber;
- driving the feed sample through the filtration system;
- creating flow conditions at each flow chamber to direct at least a portion of the viable target cell population to the first outlet, and to direct at least a portion of the nonviable cell population and at least a portion of the fluid to a second outlet;
- collecting the viable target cell population in the first collection chamber;
- and
- collecting the portion of the nonviable cell population and the portion of the fluid in the second collection chamber.
3 more elements
A method for umbilical cord blood volume reduction, the method comprising:
- introducing umbilical cord blood having an initial volume, a population of red blood cells and a viable population of a first type of nucleated cells into a filtration device within about six hours from draw, the filtration device including:
- a device inlet, and
- a flow chamber configured to separate the first type of nucleated cells from a portion of the population of red blood cells, to direct the first type of nucleated cells towards a first collection chamber, and to direct the portion of the population of red blood cells to a second collection chamber;
- passing the umbilical cord blood through the filtration device;
- collecting at least a portion of the population of red blood cells in the second collection chamber, and
- collecting at least 75% of the first type of nucleated cells in a fluid volume of less than about 25% of the initial volume in the first collection chamber, greater than about 99% of the population of nucleated cells collected in the first collection chamber being viable.
3 more elements
Dependencies are inferred from the claim text.