Gall wasp control agents
AssigneeFuturagene Israel Ltd.
InventorsDror Avisar, Hanan Stein, Ziv Shani, Daniel Siegel
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Abstract
The present invention relates to the field of RNA-mediated gene silencing in insect species. The present invention is based, in part, on the inventors' sequencing of genes from eucalyptus invasive species gall wasp pests Leptocybe invasa (Li) and Ophelimus maskelli (Om). In certain aspects, the invention provides Li and Om nucleic acids, derivatives thereof and the use of such nucleic acids and derivatives as gall wasp control agents.
Claims49
An isolated nucleic acid comprising a sequence that selectively hybridizes under high stringency hybridization conditions to the complement of a sequence selected from the group consisting of SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 45-123, SEQ ID NO:
- 127-132, SEQ ID NO:
- 234-244.
An isolated nucleic acid comprising at least 17 contiguous nucleotides of a sequence selected from the group consisting of SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25, SEQ ID NO:
- 26, SEQ ID NO:
- 45-123, SEQ ID NO:
- 127-132, SEQ ID NO:
- 234-244.
A small inhibitory ribonucleic acid molecule (siRNA) that inhibits expression of an L. invasa or O. maskelli nucleic acid molecule encoding coatamer subunit alpha (alpha COP), chromodomain helicase DNA binding protein, venom carboxylesterase 6 isoform 1, chitin synthase, ferritin or juvenile hormone epoxide hydrolase.
A double stranded ribonucleic acid molecule (dsRNA) comprising a unit of a first strand of nucleotides that is substantially identical to at least 17 contiguous nucleotides set forth in SEQ ID NO:
- 15, SEQ ID NO:
- 16, SEQ ID NO:
- 17, SEQ ID NO:
- 18, SEQ ID NO:
- 19, SEQ ID NO:
- 20, SEQ ID NO:
- 21, SEQ ID NO:
- 22, SEQ ID NO:
- 23, SEQ ID NO:
- 24, SEQ ID NO:
- 25 or SEQ ID NO:
- 26, SEQ ID NO:
- 45-123, SEQ ID NO:
- 127-132, SEQ ID NO:
- 234-244 and a second strand of nucleotides that is substantially complementary to said first strand of nucleotides.
A small inhibitory ribonucleic acid molecule (dsRNA) that inhibits expression of an essential gene of L. invasa or O. maskelli.
Dependencies are inferred from the claim text.