Compositions and Methods for the Generation of Disease Resistant Crops
OwnerDaniel F. Klessig, Hong-gu Kang +2inventors
InventorsDaniel F. Klessig, Hong-gu Kang, Karl-heinz Kogel, Patricia Manosalva
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Abstract
Compositions and methods for creating crops exhibiting enhanced pathogen resistance are disclosed.
Claims26
A method for producing a plant exhibiting increased pathogen resistance comprising,
- a) introducing a nucleic acid construct encoding RNAi specific for silencing of CRT1 and its closely related homologs into a plant cell, said RNAi effectively inhibiting CRT1 or CRT1 homolog gene expression in said plant cell, said cell exhibiting increased pathogen resistance when compared to wild type plant cells lacking said RNAi.
A nucleic acid construct encoding a CRT1 or CRT1 homolog specific RNAi which is effective to down modulate expression of said CRT1 or CRT1 homolog in a plant of interest.
A method for producing plants exhibiting increased pathogen resistance using the TILLNG method, comprising
- a) treating plant seeds with an effective amount of an agent effective to introduce mutations into the plant genome,
- b) screening the progeny plants for the presence of lesions in a CRT1 or CRT1 homolog gene, the lesions resulting in reduced production of functional CRT1 or CRT1 homolog protein,
- c) testing the plants of step b) for enhanced resistance to pathogens compared to untreated plants.
A method for producing plants exhibiting increased pathogen resistance using a transposable element system, comprising
- a) crossing, by breeding, a plant, the cells of which harbor transposon elements in their genomes near a CRT1 or CRT1 homolog gene with a plant comprising a nucleic acid which encodes an active transposase, the transposase catalyzing in the progeny plants, transposition of the transposon element into the surrounding DNA including the CRT1 or CRT1 homolog gene,
- b) screening plants so treated for the presence of lesions in said gene, the lesions being correlated with reduced production of functional CRT1 or CRT1 homolog protein,
- c) testing the plants of step b) for enhanced resistance to pathogens compared to untreated plants.
A method for producing a plant exhibiting increased pathogen resistance comprising, introducing a nucleic acid construct encoding CRT1 or its closely related homologs into a plant cell, thereby over-expressing CRT1, overexpression of CRT1 in said cell being correlated with increased pathogen resistance when compared to wild type plant cells lacking said construct, with the proviso said plant is not Arabidopsis.
A nucleic acid encoding a chimeric CRT1 protein which upon expression in a plant alters a disease resistance phenotype in said plant.
Dependencies are inferred from the claim text.